Experimental Study of the Effect of Lyophilized Platelet Growth Factors (LPGFs) on Healing of Critical Size Defect in Craniofacial Bone

Document Type : Research article

Authors

1 The Departments of Plastic, Burn & Maxillofacial Surgery, Faculty of Medicine, Ain Shams University

2 The Departments of Clinical Pathology , Faculty of Medicine, Ain Shams University

3 The Departments of Histology, Faculty of Medicine, Ain Shams University

4 The Departments of Plastic, Burn & Maxillofacial Surgery; Faculty of Medicine, Ain Shams University

Abstract

Background: The maxillofacial trauma and their complications
represent a great socio-economic concern for the
Egyptian health system. There are multiple reconstructive
options available for reconstructing the critical size defects
in craniomaxillofacial field. The reconstruction of the critical
sized defects by autografts is considered the golden standard.
They offer minimum immunological rejection, complete
histocompatibility and can provide the best osteoinductive,
osteoconductive and osteogenic properties. Cell therapy and
its applications showed great advance in reconstructive surgery
in the last years. Growth factors derived from platelet rich
plasma (PRP) can contribute to tissue regeneration in allogenic
bone graft, by assisting cell migration, proliferation, differentiation
and extra cellular matrix synthesis.
Objective: To study the effect of human lyophilized growth
factors (GFs) on healing of critical size bone defect in craniofacial
bone of animal.
Material and Methods: This study was conducted between
October 2016 and October 2018. It was conducted at the
Medical Research Center associated with Ain Shams Faculty
of Medicine and approved from the Research Ethics Committee
(REC) of Faculty of Medicine, Ain Shams University (No:
FMASU 1969/2014). Using 30 albino rats divided into 3
groups.
Results: The results obtained in this study revealed a
statistically significant bone healing after reconstruction of
critical size calvarial bone defects by Lyophilized platelet
growth factors (LPDGF) seeded on allogenic Demineralized
bone matrix (DBM). This study presents a beneficial method
for reconstruction of critical size calvarial bone defects by an
already made non-immunogenic new tissue regenerate.
Conclusion: This study advocates the use of demineralized
bone matrix (DBM) with (LPDGF) as a reconstructive tool
for bone regeneration. But further clinical studies are needed
to evaluate its rule in the unfavorable general and local
conditions.

Keywords

Main Subjects

References

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