Kamel, A., Makboul, M., Tohamy, A., Yassin, F., Ragab, E. (2018). Estimation of Stem Cell Content in Infranatant Fraction of Lipoaspirate. The Egyptian Journal of Plastic and Reconstructive Surgery, 42(1), 29-33. doi: 10.21608/ejprs.2018.214974
Assem Kamel; Mohamed Makboul; Ahmed Tohamy; Fatma Yassin; Ehab Ragab. "Estimation of Stem Cell Content in Infranatant Fraction of Lipoaspirate". The Egyptian Journal of Plastic and Reconstructive Surgery, 42, 1, 2018, 29-33. doi: 10.21608/ejprs.2018.214974
Kamel, A., Makboul, M., Tohamy, A., Yassin, F., Ragab, E. (2018). 'Estimation of Stem Cell Content in Infranatant Fraction of Lipoaspirate', The Egyptian Journal of Plastic and Reconstructive Surgery, 42(1), pp. 29-33. doi: 10.21608/ejprs.2018.214974
Kamel, A., Makboul, M., Tohamy, A., Yassin, F., Ragab, E. Estimation of Stem Cell Content in Infranatant Fraction of Lipoaspirate. The Egyptian Journal of Plastic and Reconstructive Surgery, 2018; 42(1): 29-33. doi: 10.21608/ejprs.2018.214974
Estimation of Stem Cell Content in Infranatant Fraction of Lipoaspirate
1The Department of Plastic & Reconstructive Surgery, Faculty of Medicine, Assiut University
2The Department of Histology, Faculty of Medicine, Assiut University
Abstract
Background: Stem cells are considered a powerful tool in regenerative medicine nowadays, adipose tissue is rich source of ADSCs and as product of liposuction the lipoaspirate is used to isolate ADSCs from it. Purpose: Evaluation of stem cell content, density, immunephenotyping and electron microscopic structure in the infranatant fraction of liposuction aspirate and compare the results with that obtained from fat fraction of lipoapirate using traditional method. Methods: This study was conducted on twenty-five patients from which 100 specimens was isolated those patients were attending the Outpatient Clinic of Plastic Surgery Department at Assiut University Hospital seeking body contouring between April, 2015 and September, 2016. In this study we evaluated the stem cell content in liposuction aspirate Infranatant fluid and fat from two different sites (abdomen and thigh). Results: Comparing between the range and mean of the number of viable ADSC isolated from infranatant of lipoaspirate with that isolated from fat fraction using collagenase a high statistical significance, the mean 11.47/ml while from fat fraction using collagenase was 133.08/ml. with a significant statistical difference with a p-value of 0.000 (values are per 1ml and multiplied by 104). Regarding the comparison of the means of the two harvesting sites (thigh and abdomen) by both methods we found that ADSCs was higher in the abdomen than those from the thigh but however the results were statistically insignificant with a p-value >0.05. Conclusions: Infranatat fluid of lipoaspirate is not a waste product and shouldn’t be discarded, it is a rich source of ADSCs, Time factor using infranatant fluid for stem cells is much less than that of fat using digestive enzymes and more economic.
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