Estimation of Stem Cell Content in Infranatant Fraction of Lipoaspirate

Kamel, Assem; Makboul, Mohamed; Tohamy, Ahmed; Yassin, Fatma; Ragab, Ehab

doi:10.21608/ejprs.2018.214974

Estimation of Stem Cell Content in Infranatant Fraction of Lipoaspirate

Document Type : Review Article

Authors

¹ The Department of Plastic & Reconstructive Surgery, Faculty of Medicine, Assiut University

² The Department of Histology, Faculty of Medicine, Assiut University

10.21608/ejprs.2018.214974

Abstract

Background: Stem cells are considered a powerful tool
in regenerative medicine nowadays, adipose tissue is rich
source of ADSCs and as product of liposuction the lipoaspirate
is used to isolate ADSCs from it.
Purpose: Evaluation of stem cell content, density, immunephenotyping
and electron microscopic structure in the infranatant
fraction of liposuction aspirate and compare the results
with that obtained from fat fraction of lipoapirate using
traditional method.
Methods: This study was conducted on twenty-five patients
from which 100 specimens was isolated those patients were
attending the Outpatient Clinic of Plastic Surgery Department
at Assiut University Hospital seeking body contouring between
April, 2015 and September, 2016. In this study we evaluated
the stem cell content in liposuction aspirate Infranatant fluid
and fat from two different sites (abdomen and thigh).
Results: Comparing between the range and mean of the
number of viable ADSC isolated from infranatant of lipoaspirate
with that isolated from fat fraction using collagenase a
high statistical significance, the mean 11.47/ml while from
fat fraction using collagenase was 133.08/ml. with a significant
statistical difference with a p-value of 0.000 (values are per
1ml and multiplied by 104). Regarding the comparison of the
means of the two harvesting sites (thigh and abdomen) by
both methods we found that ADSCs was higher in the abdomen
than those from the thigh but however the results were statistically
insignificant with a p-value >0.05.
Conclusions: Infranatat fluid of lipoaspirate is not a waste
product and shouldn’t be discarded, it is a rich source of
ADSCs, Time factor using infranatant fluid for stem cells is
much less than that of fat using digestive enzymes and more
economic.

Keywords

Main Subjects

New technologies and products

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