The Effect of Platelet-Rich Fibrin on Donor Site of Split Thickness Skin Graft in Burned Patients

Document Type : Research article

Authors

1 The Department of Plastic, Burn & Maxillofacial Surgery, Faculty of Medicine, Ain Shams University, Cairo, Egypt

2 The Department of Histology & Cell Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Abstract

Background: Patients with massive burn areas have now
a greater chance of survival thanks to improved modalities
of new treatments. In some patients it is necessary to harvest
multiple skin grafts from the same donor site which is restricted
by the quality of the skin and the time required to recover;
this procedure can take a long time and may increase the
danger of bacterial contamination resulting in increased
morbidity and mortality in these individuals. Platelet-rich
fibrin is one of the newest autologous growth factors utilized
for wound management. PRF application can significantly
improve the wounds' condition by supplying growth factors
to the wound environment, which aid in the epithelialization
process's acceleration, resulting in cost-effective wound
management. This will allow multiple harvesting from the
same donor site, reducing costs, hospitalization time, scars,
and co-morbidities.
Aim of the Work: To assess the role of PRF in accelerating
wound healing in the donor site following skin grafting in
burn patients.
Patients and Methods: This prospective, controlled,
clinical trial study was done from April 2020 to April 2021
included 20 patients at the burn unit of El Demerdash Hospital,
Ain Shams University.
Results: The current study showed that healing days in
donor sites injected with PRF were lower than those of
controlled areas with statistical significance and mean epidermal
thickness was lower in the control area compared to PRF
injected areas with statistical significance.
Conclusion: PRF can significantly decrease the time of
donor wound healing compared to that of conventional
treatment.

Keywords

Main Subjects

References

Reksodiputro M.H., Harba'i H.M., Koento T. and Harahap
A.R.: Platelet-rich fibrin enhances wound epithelialization
in the skin graft donor site. Journal of Physics: Conference
Series, 1073: 032046, 2018.
2- Danielsen P., Jørgensen B., Karlsmark T., Jorgensen L.N.
and Ågren M.S.J.P.: Surgery R. Effect of topical autologous
platelet-rich fibrin versus no intervention on epithelialization
of donor sites and meshed split-thickness skin autografts:
A randomized clinical trial, 122 (5): 1431-40, 2008.
3- Hotwani K. and Sharma K.: Platelet-rich fibrin-a novel
acumen into regenerative endodontic therapy. Restorative
Dentistry & Endodontics, 39 (1): 1-6, 2014.
4- Somani A. and Rai R.: Comparison of the efficacy of
autologous platelet-rich fibrin versus saline dressing in
chronic venous leg ulcers: A randomized controlled trial.
Journal of Cutaneous and Aesthetic Surgery, 10 (1): 8,
2017.
5- Sclafani A.P.: Safety, efficacy, and utility of platelet-rich
fibrin matrix in facial plastic surgery. Archives of Facial
Plastic Surgery, 13 (4): 247, 2011.
6- Singh A., Kohli M. and Gupta N.: Platelet-rich fibrin: A
novel approach for osseous regeneration. Journal of
Maxillofacial and Oral Surgery, 11 (4): 430-4, 2012.
7- Miron R.J., Fujioka-Kobayashi M., Hernandez M., Kandalam
U., Zhang Y., Ghanaati S., et al.: Injectable plateletrich
fibrin (i-PRF): Opportunities in regenerative dentistry?
Clinical Oral Investigations, 21 (8): 2619-27, 2017.
8- Suvarna S. and Layton C.: Bancroft's theory and practice
of histological techniques, 7th ed. Churchill Livingstone
Elsevier. Oxford, 2013.
9- Vaheb M., Karrabi M., Khajeh M., Asadi A., Shahrestanaki
E. and Sahebkar M.J. Tijolew: Evaluation of the effect
of platelet-rich fibrin on wound healing at split-thickness
skin graft donor sites: A randomized, placebo-controlled,
triple-blind study, 20 (1): 29-36, 2021.
10- Kakudo N., Kushida S., Minakata T., Suzuki K. and
Kusumoto K.J. Mmm: Platelet-rich plasma promotes
epithelialization and angiogenesis in a split-thickness skin
graft donor site, 44 (4): 233-6, 2011.
11- Fang Z., Yang X., Wu G., Liu M., Han J., Tao K., et al.:
The use of autologous platelet-rich plasma gel increases
wound healing and reduces scar development in splitthickness
skin graft donor sites. Journal of plastic surgery
and hand surgery, 53 (6): 356-60, 2019.
12- Ferreira L.M., Blanes L., Gragnani A., Veiga D.F., Veiga
F.P., Nery G.B., et al.: Hemicellulose dressing versus
rayon dressing in the re-epithelialization of split-thickness
skin graft donor sites: A multicenter study, 18 (3): 88-94,
2009.
13- Kaiser D., Hafner J., Mayer D., French L.E. and Läuchli
S.J. Ais: Care W. Alginate dressing and polyurethane film
versus paraffin gauze in the treatment of split-thickness
skin graft donor sites: A randomized controlled pilot study,
26 (2): 67-73, 2013.
14- Dohan Ehrenfest D.M., de Peppo G.M., Doglioli P. and
Sammartino G.J.G.F.: Slow-release of growth factors and
thrombospondin-1 in Choukroun's platelet-rich fibrin
(PRF): A gold standard to achieve for all surgical platelet
concentrates technologies, 27 (1): 63-9, 2009.
15- Dohan Ehrenfest D.M.J.O.S.: Oral medicine, oral pathology,
oral radiology, endodontics. How to optimize the
preparation of leukocyte-and platelet-rich fibrin (L-PRF,
Choukroun's technique) clots and membranes: Introducing
the PRF Box., 110 (3): 275-8, 2010.
16- Dohan D.M., Choukroun J., Diss A., Dohan S.L., Dohan
A.J., Mouhyi J., et al.: Platelet-rich fibrin (PRF): A secondgeneration
platelet concentrate. Part I: Technological
concepts and evolution. Oral surgery, oral medicine, oral
pathology, oral radiology, and endodontics, 101 (3): e37-
44, 2006.
17- Khanzadeh Alishahi M., Kazemi D., Mohajeri D., Mofidpoor
H., Afkhami Goli A., Khanzadeh Alishahi MAJIJoVS,
et al.: Histopathological evaluation of the effect of plateletrich
fibrin on canine cutaneous incisional wound healing,
5 (2): 19-32, 2013.
18- Majeed A.A. and Abood DAJVW.: Histological assessment
of the efficiency of rabbit serum in healing skin wounds,
12 (10): 1650, 2019.
19- Del Amo C., Perez-Valle A., Perez-Zabala E., Perez-del-
Pecho K., Larrazabal A., Basterretxea A., et al.: Wound
Dressing Selection Is Critical to Enhance Platelet-Rich
Fibrin Activities in Wound Care, 21 (2): 624, 2020.
20- Tsai H-C., Chang GR-L., Fan H-C., Ou-Yang H., Huang
L-C., Wu S-C., et al.: A mini-pig model for evaluating
the efficacy of autologous platelet patches on induced
acute full-thickness wound healing, 15 (1): 1-13, 2019.
21- Xu F., Zou D., Dai T., Xu H., An R., Liu Y., et al.: Effects
of incorporation of granule-lyophilized platelet-rich fibrin
into polyvinyl alcohol hydrogel on wound healing, 8 (1):
1-10, 2018.
22- Khalafı F.H. and SALIH SIJAJPCR: Clinical and histopathological
evaluation of using platelet-rich plasma
and platelet-rich fibrin matrix in the treatment of induced
chronic open wounds in bucks, 11 (5): 337-41, 2018

Files

Share

How to cite

Statistics